Difference between revisions of "McClean: Yeast Nomenclature"

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Nomenclature
 
Naming Yeast Strains
 
Now that you've become (somewhat) comfortable working with yeast in the lab,
 
it's time to master some of the jargon associated with yeast…
 
S. cerevisiae researchers have adopted a systematic approach for describing the
 
genotypes of their yeast strains. Genes that have been studied or characterized in some
 
way often have a "common" name ascribed to them, and this name generally consists of
 
three letters and a number. For example, LEU2 refers to a gene encoding an enzyme in
 
the leucine biosynthetic pathway, and PDR3 refers to a gene encoding a transcription
 
factor involved in pleiotropic drug resistance. The table below describes the basics of
 
yeast genetic nomenclature which is always typed up in italics.
 
Wild-type alleles are written in all caps LEU2
 
And sometimes with a "plus" sign LEU2+
 
Recessive mutant alleles are written in lower case arg2
 
And may include an allele number arg2-9
 
Dominant mutant alleles are written in all caps
 
And should include an allele number PDR3-11
 
Or other notation to indicate it is not wild-type. OLIr
 
Besides its "common" name, each gene has a systematic name that refers to its
 
precise position in the genome…
 
YNL323w
 
This is a yeast gene
 
On chromosome XIV (N is the 14th letter in the alphabet)
 
This gene is on the left arm of the chromosome
 
It is the 323rd gene away from the centromere
 
It is on the "Watson" strand
 
I
 

Revision as of 13:28, 4 June 2012


Overview

'S. cerevisiae' researchers have a systematic approach for describing genotypes of yeast strains. You need to follow this system when writing papers, protocols, and when entering yeast strains into the lab database.

Much of this information is taken from the "Methods in Yeast Genetics" book from Cold Spring Harbor Laboratory. See the references below.

Nomenclature

Chromosomal Genes

Nonmendelian Determinants

Genetic Background

Notes

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References

Burke, D. Dawson, D. & Sterns, T. 2000 Methods in Yeast Genetics: A Cold Spring Harbor Laboratory Course Manual Cold Spring Harbor Laboratory Press

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