McClean:Magic Marker Medias

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Revision as of 15:00, 11 October 2011 by Megan N McClean (talk | contribs) (New page: ==Overview== Medias used for doing strain construction with the magic marker technology (see references) ==10x SD/MSG== 1.7 g YNB without amino acids without ammonium sulfate (Difco, #23...)
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Overview

Medias used for doing strain construction with the magic marker technology (see references)


10x SD/MSG

1.7 g YNB without amino acids without ammonium sulfate (Difco, #233520) 1 g Monosodium glutamate (L-glutamic acid, monosodium salt MP BIomedicals #194677)

Combine YNB and monosodium glutamate in 70mls of dH2O. Dissolve the glutamate and YNB and then bring the final volume to 100mls. Autoclave. This solution is 10x.