Difference between revisions of "Lidstrom:Digestion with Restriction Enzymes"

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(Recipe)
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**If two enzymes are required, check http://www.neb.com/nebecomm/DoubleDigestCalculator.asp for buffer, temperature, BSA specifications.
 
**If two enzymes are required, check http://www.neb.com/nebecomm/DoubleDigestCalculator.asp for buffer, temperature, BSA specifications.
  
*If using BioBrick or BglBrick enzymes, chose your buffer based on Janet's notes.
+
*If using BioBrick or BglBrick enzymes, chose your buffer based on [https://docs.google.com/document/d/1j91GWl_90ZablSxtE-hFQWPhG3BHQx822zSvSH1dv04/edit Janet's notes].
  
 
== Temperature/Time ==
 
== Temperature/Time ==
 
*1 - 2 hours at 37oC or room temperature overnight
 
*1 - 2 hours at 37oC or room temperature overnight
 
*Inactivate by ..
 
*Inactivate by ..

Revision as of 08:33, 30 March 2013

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Recipe

  • If using BioBrick or BglBrick enzymes, chose your buffer based on Janet's notes.

Temperature/Time

  • 1 - 2 hours at 37oC or room temperature overnight
  • Inactivate by ..