IGEM:UW/2008/Notebook/iGEM UW Team/2008/06/19: Difference between revisions
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(Autocreate 2008/06/19 Entry for IGEM:UW/2008/Notebook/iGEM_UW_Team) |
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Primers for fusion of protein A and ompA and alpha or omega part of beta-lactamase were designed: | |||
alphaL+link14+homo CAGTGGTGGAAGCGGTGGC GGAGGATCTGGAGGAGGTGGTTCTGGAGGTGGAGGT CACCCAGAAACGCTGGTGAAAG | |||
alphaP+STOP+XbaI+BamHI GG GGATCC GG TCTAGA TTA TTCGCCAGTTAATAGTTTGCG | |||
omegaL+link14+homo CAGTGGTGGAAGCGGTGGC GGAGGATCTGGAGGAGGTGGTTCTGGAGGTGGAGGT CTACTTACTCTAGCTTCCCGG | |||
omegaP+STOP+EcoRI+PstI+BamHI AAGGATCCAACTGCAGAAGAATTC TTACCAATGCTTAATCAGTG | |||
OmpAL+NdeI CATATGAAAGCTACTAAACTGGTACTGGG | |||
OmpAP+link8+SacI+NotI+homo GCCACCGCTTCCACCACT GCGGCCGC CA GAGCTC CCTCCTCCAGAACCTCC GCCGTTGTCCGGACGAGTGCCGATG | |||
Revision as of 11:22, 26 June 2008
 University of Warsaw iGEM Team 2008
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Primers for fusion of protein A and ompA and alpha or omega part of beta-lactamase were designed: alphaL+link14+homo CAGTGGTGGAAGCGGTGGC GGAGGATCTGGAGGAGGTGGTTCTGGAGGTGGAGGT CACCCAGAAACGCTGGTGAAAG alphaP+STOP+XbaI+BamHI GG GGATCC GG TCTAGA TTA TTCGCCAGTTAATAGTTTGCG omegaL+link14+homo CAGTGGTGGAAGCGGTGGC GGAGGATCTGGAGGAGGTGGTTCTGGAGGTGGAGGT CTACTTACTCTAGCTTCCCGG omegaP+STOP+EcoRI+PstI+BamHI AAGGATCCAACTGCAGAAGAATTC TTACCAATGCTTAATCAGTG OmpAL+NdeI CATATGAAAGCTACTAAACTGGTACTGGG OmpAP+link8+SacI+NotI+homo GCCACCGCTTCCACCACT GCGGCCGC CA GAGCTC CCTCCTCCAGAACCTCC GCCGTTGTCCGGACGAGTGCCGATG
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