IGEM:UW/2008/Notebook/iGEM UW Team/2008/06/19

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Primers for fusion of protein A and ompA and alpha or omega part of beta-lactamase were designed:

alphaL+link14+homo CAGTGGTGGAAGCGGTGGC GGAGGATCTGGAGGAGGTGGTTCTGGAGGTGGAGGT CACCCAGAAACGCTGGTGAAAG

alphaP+STOP+XbaI+BamHI GG GGATCC GG TCTAGA TTA TTCGCCAGTTAATAGTTTGCG

omegaL+link14+homo CAGTGGTGGAAGCGGTGGC GGAGGATCTGGAGGAGGTGGTTCTGGAGGTGGAGGT CTACTTACTCTAGCTTCCCGG

omegaP+STOP+EcoRI+PstI+BamHI AAGGATCCAACTGCAGAAGAATTC TTACCAATGCTTAATCAGTG

OmpAL+NdeI CATATGAAAGCTACTAAACTGGTACTGGG

OmpAP+link8+SacI+NotI+homo GCCACCGCTTCCACCACT GCGGCCGC CA GAGCTC CCTCCTCCAGAACCTCC GCCGTTGTCCGGACGAGTGCCGATG