Difference between revisions of "IGEM:UW/2008/Notebook/iGEM UW Team/2008/05/23"

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11. Transformants plating on LB + tetracycline
 
11. Transformants plating on LB + tetracycline
  
[[Image: UW_A+T.jpg|350px]]
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[[Image: UW_A+T.jpg|350px]] [[Image: UW_AT.jpg.jpg|350px]]
  
  

Revision as of 10:41, 12 June 2008

Igem-logo-150px.png University of Warsaw iGEM Team 2008 <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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1. Digest of pMPM-T5 with NcoI and XbaI

2. Digest of PCR product for translation fusion (AID+T7 RNA-polimerase) with NcoI and XbaI

3. Clean-up of digested products from p.1 and p.2

4. Ligation of digested products from p.1 and p.2

5. Transformation of E.coli TOP10 with ligation product

6. Digest of pMPM-T5+AID with HindIII and SalI

7. Digest of PCR product for transcription fusion (T7 RNA-polimerase) with HindIII and SalI

8. Clean-up of digested products from p.6 and p.7

9. Ligation of digested products from p.6 and p.7

10. Transformation of E.coli TOP10 with ligation product

11. Transformants plating on LB + tetracycline

UW A+T.jpg 350px