Difference between revisions of "IGEM:PennState/2007/Progress Cloning"

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(New page: Primers: <h2>Promoter Region Primers</h2> F-GGAATTCGCGGCCGCTTCTAGAG *TmC R-CTGCAGCGGCCGCTACTAGTA *TmC <h3>Double Promoter</h3> Full Promoter AtoF F-GGAATTCGCGGCCGCTTCTAGAGTATTGAACTCCATA...)
 
 
Line 1: Line 1:
 
Primers:
 
Primers:
 
<h2>Promoter Region Primers</h2>
 
<h2>Promoter Region Primers</h2>
 
F-GGAATTCGCGGCCGCTTCTAGAG
 
*TmC
 
R-CTGCAGCGGCCGCTACTAGTA
 
*TmC
 
  
 
<h3>Double Promoter</h3>
 
<h3>Double Promoter</h3>
Line 50: Line 45:
 
*TmC 57
 
*TmC 57
  
<h3>XylR</h3>
+
<h2>XylR</h2>
 +
 
 +
XylR with Promoter RBS and terminator: Right
 +
F-GGAATTCGCGGCCGCTTCTAGAGGCAACCAAACGCCGTTCTTG
 +
*TmC 65
 +
R-CTGCAGCGGCCGCTACTAGTAATTGCATTTCCTTGAGCCTTATCC
 +
*TmC 63
 +
 
 +
XylR with Promoter RBS and terminator: Left
 +
F-GGAATTCGCGGCCGCTTCTAGAGATTGCATTTCCTTGAGCCTTATCC
 +
*TmC 63
 +
R-CTGCAGCGGCCGCTACTAGTAGCAACCAAACGCCGTTCTTG
 +
*TmC 65
 +
 
 +
XylR ORF Right
 +
**Missing**
 +
 
 +
XylR ORF Left
 +
**Missing**

Latest revision as of 11:27, 12 June 2007

Primers:

Promoter Region Primers

Double Promoter

Full Promoter AtoF F-GGAATTCGCGGCCGCTTCTAGAGTATTGAACTCCATAATCAGGTAATG

  • TmC 61

R-CTGCAGCGGCCGCTACTAGTATGGTGTAGGGCCTTCTGTAG

  • TmC 62

Full Promoter FtoA F-GGAATTCGCGGCCGCTTCTAGAGTGGTGTAGGGCCTTCTGTA

  • TmC 61

R-CTGCAGCGGCCGCTACTAGTATATTGAACTCCATAATCAGGTAATG

  • TmC 58

ORF Promoter AtoF

    • MISSING**

ORF Promoter FtoA F-GGAATTCGCGGCCGCTTCTAGAGAACCAATTTTTAGCAACTAAACAG

  • TmC 58

R-CTGCAGCGGCCGCTACTAGTAATGGATGATGTCGTAATATTGG

  • TmC 59

Single Promoter

CRP XylR XylR Fis F- GGAATTCGCGGCCGCTTCTAGAGTCCCTTTCAGTTGCTCAAT

  • TmC 58

R- CTGCAGCGGCCGCTACTAGTAGGTGTAGGGCCTTCTGTAG

  • TmC 60

XYLR XYLR FIS F-GGAATTCGCGGCCGCTTCTAGAGTTACGTTTATCGCGGTGATT

  • TmC 59

R-CTGCAGCGGCCGCTACTAGTAGGTGTAGGGCCTTCTGTAG

  • TmC 60

CRP

F-GGAATTCGCGGCCGCTTCTAGAGATGGTGCTTGGCAAACC

  • TmC 60

R-CTGCAGCGGCCGCTACTAGTATTAACGAGTGCCGTAAAC

  • TmC 57

XylR

XylR with Promoter RBS and terminator: Right F-GGAATTCGCGGCCGCTTCTAGAGGCAACCAAACGCCGTTCTTG

  • TmC 65

R-CTGCAGCGGCCGCTACTAGTAATTGCATTTCCTTGAGCCTTATCC

  • TmC 63

XylR with Promoter RBS and terminator: Left F-GGAATTCGCGGCCGCTTCTAGAGATTGCATTTCCTTGAGCCTTATCC

  • TmC 63

R-CTGCAGCGGCCGCTACTAGTAGCAACCAAACGCCGTTCTTG

  • TmC 65

XylR ORF Right

    • Missing**

XylR ORF Left

    • Missing**