IGEM:Peking/2007/Switch-Notebook/2007-7-13
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Experimenter: CCY, CDZ
Contents
Precipitate with ethanol: SDA-H, Y, Cro_lacZa
由于缺少pCC002^^ (NotI/XhoI), 无法做连接 做载体!
Miniprep and digestion test: SDD-EGFP-pCC002(1-15)
l to r: Marker, SDD-EGFP-pCC002(1-15)
Save: SDD-EGFP-pCC002(1,5,7,8,10)
pLX007-lacZa用lacZa-F/test-R, test-F/lacZa-R对切
Failed!
只好大批摇菌提质粒。一共53个!!!!!