Difference between revisions of "IGEM:MIT/2007/Protocols"

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(DNA Work)
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*[[../Notebook/2007-6-13#Ligation|Ligation]]
 
*[[../Notebook/2007-6-13#Ligation|Ligation]]
 
*[[../Notebook/2007-6-13#Purifying_the_DNA|Purifying the DNA]]
 
*[[../Notebook/2007-6-13#Purifying_the_DNA|Purifying the DNA]]
 +
*[[../Notebook/2007-6-14#Transformation_Protocol|Transformation]]

Revision as of 06:58, 14 June 2007

General Lab Protocols

  • [[../Notebook/2007-6-11#General_Lab_Do_s_and_Don’t_s|General Lab Do's and Don'ts]]
  • [[../Notebook/2007-6-12#Nanodrop (~20ng/ul) Protocol|Calculating Optical Density; Nanodrop Protocol]]
  • [[../Notebook/2007-6-11#made_1_liter_1%_agarose_solution|Making Agarose Solution]]
  • [[../Notebook/2007-6-13#Plating_on_Agar|Plating on Agar]]
  • [[../Notebook/2007-6-11#innoculate_5ml_LB/Amp_with_bact._colonies_(EGFP_and_NNX)|Innoculating LB with bacteria]]


DNA Work

  • [[../Notebook/2007-6-12#PCR_amplification_of_EGFP|PCR Amplification]]
  • [[../Notebook/2007-6-12#PCR_purify(microcentrifuge)|PCR Purification in a Microcentrifuge]]
  • [[../Notebook/2007-6-13#Ligation|Ligation]]
  • [[../Notebook/2007-6-13#Purifying_the_DNA|Purifying the DNA]]
  • [[../Notebook/2007-6-14#Transformation_Protocol|Transformation]]