Difference between revisions of "IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/07/30"

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(Team Flavor)
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==Team Allergy==
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*Sequencing Results:
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LTP ihpRNA parts that worked: 9c2, 11c1, 11c2
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Bet 2 ihpRNA parts that worked: 25c1,c2, 28c1,(LTPS+PDK is actually Bet 2 hpRNA)
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Ger ihpRNA parts that worked: 36c1,c2, 37c1,2, 38c1,c2
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==Team Flavor==
 
==Team Flavor==
 
* Ligated StrepII tagged proteins into V24 backbone
 
* Ligated StrepII tagged proteins into V24 backbone

Revision as of 11:29, 30 July 2010

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Team Allergy

  • Sequencing Results:

LTP ihpRNA parts that worked: 9c2, 11c1, 11c2 Bet 2 ihpRNA parts that worked: 25c1,c2, 28c1,(LTPS+PDK is actually Bet 2 hpRNA) Ger ihpRNA parts that worked: 36c1,c2, 37c1,2, 38c1,c2

Team Flavor

  • Ligated StrepII tagged proteins into V24 backbone
  • Ligated cut J45004 into V0120 backbone

Ligations were plated and left @ 37°C O/N.

Team Fence

PCR of VP16 and Gal4DBD

PCR VP16 Gal4DBD 7-30.jpg