Haynes Lab:Notebook/Investigating Photo-Switchable Synthetic Nucleosomes/2012/11/21: Difference between revisions
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| <u>Reagent</u> || <u>Volume</u> | | <u>Reagent</u> || <u>Volume</u> | ||
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| DNA (clean digest) || | | DNA (clean digest) || 11.0 (~200 ng) | ||
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| 10x buffer d.p. || 1.5 | | 10x buffer d.p. || 1.5 |
Revision as of 14:37, 23 November 2012
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11/21/12Results from yesterday's ligation
PCR program:
Assemblies
> Dephosphorylation (Roche)
Proceed directly to Transformation. See http://openwetware.org/wiki/Haynes:Assembly101
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