In a 30 μL reaction, cut a pSB1A2 plasmid (from Rene) XbaI and SpeI, then ran on 1% gel and used Zymoclean Gel DNA Recovery Kit
Reagent
pSB1A2
DNA
15.0
XbaI
1.0
SpeI
1.0
10x buffer
3.0
dH2O
10.0
Total vol.
30.0
Results of the enzyme digest is shown, with both pSB1A3 bands on the right. The gel was loaded with 30 μL samples. The bands that were cut are outlined in green.