Haynes Lab:Notebook/Engineering PC-TFs/2013/09/26: Difference between revisions
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==Summary== | ==Summary== | ||
*Perform restriction digest for CMV promoter | |||
{| {{table}} cellspacing="3" <!-- Digest rxn. table --> | |||
|- valign="top" | |||
| bgcolor=#cfcfcf | '''Reagent''' | |||
| bgcolor=#cfcfcf | '''Volume''' | |||
|- | |||
| DNA (plasmid) || 10 μL | |||
|- | |||
| XbaI|| 1.0 μL | |||
|- | |||
| PstI|| 1.0 μL | |||
|- | |||
| Green 10x FastDigest buffer || 3.0 μL | |||
|- | |||
| dH<sub>2</sub>O || 15 μL | |||
|- | |||
| || 30 μL --> 37°C/ ~10 min. | |||
|} | |||
*Measure the following into 1.5 mL tubes. | *Measure the following into 1.5 mL tubes. | ||
{| {{table}} cellspacing="3" <!-- Digest rxn. table --> | {| {{table}} cellspacing="3" <!-- Digest rxn. table --> |
Revision as of 19:15, 26 September 2013
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Summary
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