Difference between revisions of "Haynes Lab:Notebook/Engineering PC-TFs/2013/09/26"

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(Summary)
(Summary)
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==Summary==
 
==Summary==
 +
*Perform restriction digest for CMV promoter
 +
 +
{| {{table}} cellspacing="3" <!-- Digest rxn. table -->
 +
|- valign="top"
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| bgcolor=#cfcfcf | '''Reagent'''
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| bgcolor=#cfcfcf | '''Volume'''
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|-
 +
| DNA (plasmid) || 10 μL
 +
|-
 +
| XbaI|| 1.0 μL
 +
|-
 +
| PstI|| 1.0 μL
 +
|-
 +
| Green 10x FastDigest buffer || 3.0 μL
 +
|-
 +
| dH<sub>2</sub>O || 15 μL
 +
|-
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| &nbsp; || 30 μL --> 37°C/ ~10 min.
 +
|}
 +
 +
 
*Measure the following into 1.5 mL tubes.
 
*Measure the following into 1.5 mL tubes.
 
{| {{table}} cellspacing="3" <!-- Digest rxn. table -->
 
{| {{table}} cellspacing="3" <!-- Digest rxn. table -->

Revision as of 19:15, 26 September 2013


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Summary

  • Perform restriction digest for CMV promoter
Reagent Volume
DNA (plasmid) 10 μL
XbaI 1.0 μL
PstI 1.0 μL
Green 10x FastDigest buffer 3.0 μL
dH2O 15 μL
  30 μL --> 37°C/ ~10 min.


  • Measure the following into 1.5 mL tubes.
Reagent 15% 10% 7% 5% 2%
30% PEG 50 μL 33 μL 23 μL 17 μL 7 μL
DNA 15μL 15 μL 15 μL 15 μL 15 μL
dH2O 35 μL 52 μL 62 μL 68 μL 78 μL
Total 100 μL 100 μL 100 μL 100 μL 100 μL