Difference between revisions of "Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/03/07"

From OpenWetWare
Jump to: navigation, search
(Entry title)
(Entry title)
Line 29: Line 29:
  
 
transformed RBS+LuxI and RBS+LasI
 
transformed RBS+LuxI and RBS+LasI
 +
 +
{| {{table}}
 +
|-
 +
|Part||260||280||260/280||ng/ul
 +
|-
 +
|K079021||0.131||0.07||1.836||130.892
 +
|-
 +
|I13522||0.125||0.068||125.239
 +
|-
 +
|K079020||0.086||0.046||1.855||85.913
 +
|-
 +
|J45200||0.081||0.045||1.81||80.868
 +
|-
 +
|J04450||0.071||0.038||1.859||70.782
 +
|-
 +
|}
  
  

Revision as of 13:34, 28 May 2013

Owwnotebook icon.png Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Entry title

minipreps

streak

cut and extract and clone

pull out promoters + RBS from registry

gel extract and ligate and transform part 6


miniprep C0061 1 and 2 C0062 K081016 that haynes grew up, streaked on plate (she found by PCR) R0071 that haynes grew up, streaking on plate " "

cut K081016 and R0071 from above, ran on a gel, didn't work

Ernesto ran colony PCR on a bunch of mini preps and colonies

transformed RBS+LuxI and RBS+LasI

Part 260 280 260/280 ng/ul
K079021 0.131 0.07 1.836 130.892
I13522 0.125 0.068 125.239
K079020 0.086 0.046 1.855 85.913
J45200 0.081 0.045 1.81 80.868
J04450 0.071 0.038 1.859 70.782