Gill:TNA (Tryptone Nutrient Agar)

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Revision as of 11:42, 23 April 2013 by Jacqueline K. Grimm (talk | contribs) (New page: ==Materials== 500 mL: *Tryptone 2.5 g *Yeast extract 1.25 g *Glucose 0.5 g *NaCl 4.25 g *Agar 7.5 g *dH<sub>2</sub>O 500 ml ==Procedure== Mix all ingredients in a 1 litre bott...)
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Materials

500 mL:

  • Tryptone 2.5 g
  • Yeast extract 1.25 g
  • Glucose 0.5 g
  • NaCl 4.25 g
  • Agar 7.5 g
  • dH2O 500 ml

Procedure

Mix all ingredients in a 1 litre bottle or flask, cover and autoclave. Label caps with “TNA” to avoid confusion. Immediately after autoclaving, swirl media to dissolve the melted agar. Tighten caps and place bottles in the water bath. Pour plates when the agar temperature is 50-60 ºC.

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!