Gill:Lambda diluent (SM buffer): Difference between revisions
From OpenWetWare
Jump to navigationJump to search
mNo edit summary |
No edit summary |
||
Line 11: | Line 11: | ||
|5.8 g (100 mM) | |5.8 g (100 mM) | ||
|- | |- | ||
| | |MgSO<sub>4</sub> | ||
|1 g (8 mM) | |1 g (8 mM) | ||
|- | |- | ||
Line 17: | Line 17: | ||
|5 ml (0.01 %) | |5 ml (0.01 %) | ||
|- | |- | ||
| | |dH<sub>2</sub>O | ||
|475 ml | |475 ml | ||
|} | |} |
Latest revision as of 14:02, 23 April 2013
Materials
1000 mL | |
---|---|
1M Tris-HCl, pH 7.5 | 50 ml (25 mM) |
NaCl | 5.8 g (100 mM) |
MgSO4 | 1 g (8 mM) |
Gelatin (2% sol’n) | 5 ml (0.01 %) |
dH2O | 475 ml |
Procedure
Mix all ingredients, then aliquot 100 ml into square milk bottles & autoclave. Let bottles cool and tighten caps, store at room temp.
Notes
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!