Difference between revisions of "Gill:Lambda diluent (SM buffer)"

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m
 
Line 11: Line 11:
 
|5.8 g (100 mM)
 
|5.8 g (100 mM)
 
|-
 
|-
|MgSO4
+
|MgSO<sub>4</sub>
 
|1 g (8 mM)
 
|1 g (8 mM)
 
|-
 
|-
Line 17: Line 17:
 
|5 ml (0.01 %)
 
|5 ml (0.01 %)
 
|-
 
|-
|dH2O
+
|dH<sub>2</sub>O
 
|475 ml
 
|475 ml
 
|}
 
|}

Latest revision as of 14:02, 23 April 2013

Materials

1000 mL
1M Tris-HCl, pH 7.5 50 ml (25 mM)
NaCl 5.8 g (100 mM)
MgSO4 1 g (8 mM)
Gelatin (2% sol’n) 5 ml (0.01 %)
dH2O 475 ml

Procedure

Mix all ingredients, then aliquot 100 ml into square milk bottles & autoclave. Let bottles cool and tighten caps, store at room temp.

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!