Difference between revisions of "DMPC"

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DMPC is an acronym for di-methyl-propyl carbonate.  It is typically used to treat water to remove RNases.
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[[Image:MolecularFormulaDMPC.gif|thumb|dimethyl pyrocarbonate]]
  
DMPC reacts with amine, hydroxy and thiol groups of proteins (such as RNAses) and inactivates RNAses. Treatment involves adding DMPC to 0.1% v/v and incubating at 37°C for 1 hour to overnight followed by autoclaving. Autoclaving destroys DMPC and is an essential step.  Esters may be generated during autoclaving giving rise to a 'fruity' smell (that is not coming directly from DMPC).
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DMPC is an acronym for di-methyl pyrocarbonate (AKA: dimethyl dicarbonate, pyrocarbonic acid dimethyl ester).  
  
Note that DMPC cannot be used with chemical solutions that have amine groups, such as Tris and HEPES buffers, or mercaptans.  In such cases, use DMPC-treated water to generate the solution.
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==Purpose==
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*DMPC is typically used to treat water to remove RNases. It reacts with amine, hydroxy and thiol groups of proteins (such as RNAses) and inactivates RNAses.
  
DMPC is a safer alternative to [[DEPC]] (known carcinogen). It is used in exactly the same way.
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==Procurement==
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*You can buy DMPC for example at [http://www.sigmaaldrich.com Sigma].
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==Use==
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*DMPC is a safer alternative to [[DEPC]] (known carcinogen). It is used in exactly the same way.
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*Treatment involves adding DMPC to 0.1% v/v and incubating at 37°C for 1 hour to overnight followed by autoclaving. Autoclaving destroys DMPC and is an essential step. Esters may be generated during autoclaving giving rise to a 'fruity' smell (that is not coming directly from DMPC).
 +
 
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*Note that DMPC cannot be used with chemical solutions that have amine groups, such as Tris and HEPES buffers, or mercaptans. In such cases, use DMPC-treated water to generate the solution.
 +
 
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==Safety==
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*DMPC is a safer alternative to [[DEPC]] (known carcinogen).
  
 
==References==
 
==References==
 
#[http://stanxterm.aecom.yu.edu/wiki/index.php?page=RNA_handling Handling RNA]
 
#[http://stanxterm.aecom.yu.edu/wiki/index.php?page=RNA_handling Handling RNA]
 
#[http://www.ambion.com/techlib/tb/tb_178.html RNase and DEPC Treatment: Fact or Laboratory Myth]
 
#[http://www.ambion.com/techlib/tb/tb_178.html RNase and DEPC Treatment: Fact or Laboratory Myth]
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[[Category:Material]]
 
[[Category:Material]]
 
[[Category:RNA]]
 
[[Category:RNA]]
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[[Category:Chemical]]

Latest revision as of 13:29, 3 August 2011

dimethyl pyrocarbonate

DMPC is an acronym for di-methyl pyrocarbonate (AKA: dimethyl dicarbonate, pyrocarbonic acid dimethyl ester).

Purpose

  • DMPC is typically used to treat water to remove RNases. It reacts with amine, hydroxy and thiol groups of proteins (such as RNAses) and inactivates RNAses.

Procurement

  • You can buy DMPC for example at Sigma.

Use

  • DMPC is a safer alternative to DEPC (known carcinogen). It is used in exactly the same way.
  • Treatment involves adding DMPC to 0.1% v/v and incubating at 37°C for 1 hour to overnight followed by autoclaving. Autoclaving destroys DMPC and is an essential step. Esters may be generated during autoclaving giving rise to a 'fruity' smell (that is not coming directly from DMPC).
  • Note that DMPC cannot be used with chemical solutions that have amine groups, such as Tris and HEPES buffers, or mercaptans. In such cases, use DMPC-treated water to generate the solution.

Safety

  • DMPC is a safer alternative to DEPC (known carcinogen).

References

  1. Handling RNA
  2. RNase and DEPC Treatment: Fact or Laboratory Myth