Difference between revisions of "Chemical transformation buffer comparison"

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Line 73: Line 73:
 
|-
 
|-
 
|FSB
 
|FSB
|100mM
+
|
 
|10mM
 
|10mM
 
|10mM
 
|10mM
Line 80: Line 80:
 
|3mM
 
|3mM
 
|8%
 
|8%
|2x 3.5%
+
|8%
|5%
+
|
 
|
 
|
 
|pH 6.4->6.2
 
|pH 6.4->6.2
|Invitrogen '525
+
|Stratagene '525
 
|-
 
|-
 
|CCMB80
 
|CCMB80

Revision as of 09:33, 2 May 2006

Buffer KCl KCH3COO CaCl2 MnCl2 RbCl HACoCl3 Glycerol DMSO Sucrose Other pH Reference
SF 100mM 10mM 50mM 10% pH 6.1 Hanahan patent
RFT 30mM 10mM 50mM 100mM 15% pH 5.8 Hanahan patent
FSB 100mM 10mM 45mM 3mM 10% w/v pH 6.2 Hanahan patent
FSB 100mM 10mM 10mM 44mM ? 3mM 10% 2x 3.5% 5% pH 6.4->6.2 Jessee patent
FSB 10mM 10mM 45mM 3mM 8% 8% pH 6.4->6.2 Stratagene '525
CCMB80 10mM 80mM 20mM 10% 7% pH 6.4 Invitrogen '464
TB 250mM 15mM 15mM 7% pH 5.7->6.2 Inoue90