ChIP-Chip E. coli
This protocol is in developement and currently under investigation in the lab. Please contibute and watch changes in the next weeks!
Torsten Waldminghaus 06:29, 17 March 2008 (CDT)
Anyone should feel free to add themselves as a curator for this consensus protocol. You do not need to be a curator in order to contribute. The OpenWetWare community is currently discussing the idea of protocol curators. Please contribute.
ChIP-Chip stands for chromatine immuno precipitation and Chip in the sence of DNA-array. It is a technique to determin the DNA binding sites of a DNA binding protein on a global scale. While the basic priciple is the same there are some differences in handling cells from various organisms. This protocol is developed and tested for E. coli. It should work the same way for other bacteria but that remains to be proven (please add any experience). The main difference to other protocols is that no amplification is done after reversion of cross-linking. The basic procedure is described in .
Other ChIP-Chip protocols can be foun here:
List everything necessary to perform the protocol here. Include all information about suppliers, ordering details, etc. Links to the suppliers' page on that material are also appropriate and encouraged. Please be aware that users of this protocol may not be working in the same country as you.
Biological resources e.g. cell lines, buffers (link to a method for making them), enzymes, chemicals, kits, etc.
Any equipment used to perform the protocol (link to a method for using them).
A step by step guide to the experimental procedure.
Referenced from the main protocol, a more thorough explanation of particularly important steps in the protocol.
Referenced from the main protocol, an explanation of what can cause things to go wrong with the protocol.
Referenced from the main protocol, any comments about the protocol should be made here; i.e. how it was developed. Any comments added should be signed (by adding *'''~~~~''': in front) and explained. Links to FAQs/tips provided by other sources such as the manufacturer or other websites would be best made here.
Anecdotal observations that might be of use to others can also be posted here; e.g. 'my cells were still floating'.
It might also be good to add an image to show the workflow and timescales for experiment planning.
Acnkowledge any help you had in development, testing, writing this protocol.
- Grainger DC, Hurd D, Goldberg MD, and Busby SJ. Association of nucleoid proteins with coding and non-coding segments of the Escherichia coli genome. Nucleic Acids Res. 2006;34(16):4642-52. DOI:10.1093/nar/gkl542 |
Add links to all the OWW protocols that have been used in making the consensus.
You can discuss this protocol.
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