Difference between revisions of "Cfrench:primerlist"

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(New page: ==Catalog of primers== ===Primers for iGEM2007 flavours/fragrances project=== '''echf1''' aat gaattc gcggccgc t tctag atg agc aaa tac gaa ggt c '''Purpose''': forward primer for ''ech (...)
 
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'''echf1''' aat gaattc gcggccgc t tctag atg agc aaa tac gaa ggt c
 
'''echf1''' aat gaattc gcggccgc t tctag atg agc aaa tac gaa ggt c
 
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*Purpose: forward primer for ''ech (ferA)'' coding sequence from ''Ps. fluorescens'' NCIMB 9046.
'''Purpose''': forward primer for ''ech (ferA)'' coding sequence from ''Ps. fluorescens'' NCIMB 9046.
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*Restriction sites: EcoRI, XbaI
 
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*Notes: no ribosome binding site included.
'''Restriction sites''': EcoRI, XbaI
 
  
 
'''echr1''' ct actagt a tta tta gcg ttt ata ggc ttg cag c
 
'''echr1''' ct actagt a tta tta gcg ttt ata ggc ttg cag c
 
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*Purpose: reverse primer for ''ech (ferA)'' coding sequence from ''Ps. fluorescens'' NCIMB 9046.
'''Purpose''': reverse primer for ''ech (ferA)'' coding sequence from ''Ps. fluorescens'' NCIMB 9046.
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*Restriction sites: SpeI only.
 
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*Notes: replaces TGA stop codon with TAA TAA.
'''Restriction sites''': SpeI only.
 
 
 
'''Notes''': replaces TGA stop codon with TAA TAA.
 
  
 
[[French_Lab|Back to Main Page]]
 
[[French_Lab|Back to Main Page]]

Revision as of 03:37, 23 July 2007

Catalog of primers

Primers for iGEM2007 flavours/fragrances project

echf1 aat gaattc gcggccgc t tctag atg agc aaa tac gaa ggt c

  • Purpose: forward primer for ech (ferA) coding sequence from Ps. fluorescens NCIMB 9046.
  • Restriction sites: EcoRI, XbaI
  • Notes: no ribosome binding site included.

echr1 ct actagt a tta tta gcg ttt ata ggc ttg cag c

  • Purpose: reverse primer for ech (ferA) coding sequence from Ps. fluorescens NCIMB 9046.
  • Restriction sites: SpeI only.
  • Notes: replaces TGA stop codon with TAA TAA.

Back to Main Page