Cfrench:primerlist: Difference between revisions
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(New page: ==Catalog of primers== ===Primers for iGEM2007 flavours/fragrances project=== '''echf1''' aat gaattc gcggccgc t tctag atg agc aaa tac gaa ggt c '''Purpose''': forward primer for ''ech (...) |
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'''echf1''' aat gaattc gcggccgc t tctag atg agc aaa tac gaa ggt c | '''echf1''' aat gaattc gcggccgc t tctag atg agc aaa tac gaa ggt c | ||
*Purpose: forward primer for ''ech (ferA)'' coding sequence from ''Ps. fluorescens'' NCIMB 9046. | |||
*Restriction sites: EcoRI, XbaI | |||
*Notes: no ribosome binding site included. | |||
'''echr1''' ct actagt a tta tta gcg ttt ata ggc ttg cag c | '''echr1''' ct actagt a tta tta gcg ttt ata ggc ttg cag c | ||
*Purpose: reverse primer for ''ech (ferA)'' coding sequence from ''Ps. fluorescens'' NCIMB 9046. | |||
*Restriction sites: SpeI only. | |||
*Notes: replaces TGA stop codon with TAA TAA. | |||
[[French_Lab|Back to Main Page]] | [[French_Lab|Back to Main Page]] | ||
Revision as of 03:37, 23 July 2007
Catalog of primers
Primers for iGEM2007 flavours/fragrances project
echf1 aat gaattc gcggccgc t tctag atg agc aaa tac gaa ggt c
- Purpose: forward primer for ech (ferA) coding sequence from Ps. fluorescens NCIMB 9046.
- Restriction sites: EcoRI, XbaI
- Notes: no ribosome binding site included.
echr1 ct actagt a tta tta gcg ttt ata ggc ttg cag c
- Purpose: reverse primer for ech (ferA) coding sequence from Ps. fluorescens NCIMB 9046.
- Restriction sites: SpeI only.
- Notes: replaces TGA stop codon with TAA TAA.
