BME103:T930 Group 3 l2: Difference between revisions

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<!--- A description of the diseases and their associated SNP's (include the database reference number and web link) --->
<!--- A description of the diseases and their associated SNP's (include the database reference number and web link) --->
1.) The amyloid Beta precursor protein for Alzheimer's - <br>
1.) The amyloid Beta precursor protein for Alzheimer's - <br>
rs63751263 (http://omim.org/entry/104760#0008) <br>
rs63751263 (http://omim.org/entry/104760#0008) <br>
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<!--- Include the sequences of your forward and reverse primers. Explain why a disease allele will give a PCR product and the non-disease allele will not. --->
<!--- Include the sequences of your forward and reverse primers. Explain why a disease allele will give a PCR product and the non-disease allele will not. --->
1.) Forward primer: CTTC''[G]''ACCT<br>
1.) Forward primer: CTTC''[G]''ACCT<br>
Reverse primer: TCCA''[G]''CTTC <br><br>
Reverse primer: TCCA''[G]''CTTC <br><br>
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<!--- Include an illustration that shows how your system's primers allow specific amplification of the disease-related SNP --->
<!--- Include an illustration that shows how your system's primers allow specific amplification of the disease-related SNP --->
[[Image:DNA Amplification.jpg|200px|Exponential amplification of a specific gene.]]<br>
[[Image:DNA Amplification.jpg|200px|Exponential amplification of a specific gene.]]<br>


Revision as of 23:01, 22 November 2012

BME 103 Fall 2012 Home
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Lab Write-Up 1
Lab Write-Up 2
Lab Write-Up 3
Course Logistics For Instructors
Photos
Wiki Editing Help

OUR TEAM

Name: Lekha Anantuni
Role: R&D
Name: Rohan Kumar
Role(s)
Name: Kyle Stoneking
Role(s)
Name: Austin Cuaderno
Role(s)
Name: Josh Eger
Role(s)

LAB 2 WRITE-UP

Thermal Cycler Engineering

Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.


System Design


Key Features


Instructions





Protocols

Materials


PCR Protocol



DNA Measurement Protocol



Research and Development

Background on Disease Markers

1.) The amyloid Beta precursor protein for Alzheimer's -
rs63751263 (http://omim.org/entry/104760#0008)
AGACGGAGGAGATCTCTGAAGTGAAG [A/C] TGGATGCAGAATTCCGACATGACTC

2.) Ubiquitin-like Modifier-activating enzyme for Spinal muscular atrophy -
rs80356547(http://omim.org/entry/314370#0002)
GATGGCGTGGCCAATGCCCTGGACAA [C/T] GTCCATGCCCGTCAGTTTGGAGGCG

Primer Design

1.) Forward primer: CTTC[G]ACCT
Reverse primer: TCCA[G]CTTC

2.) Forward primer: TGTT[A]CAGG
Reverse primer: GGAC[A]TTGT


Illustration

Exponential amplification of a specific gene.


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