BME100 f2013:W1200 Group1 L4: Difference between revisions
Line 62: | Line 62: | ||
'''DNA Sample Set-up Procedure''' | '''DNA Sample Set-up Procedure''' | ||
# Step 1 | # Step 1: Fill each reaction tube with 50μL of the PCR reaction mix and label all tubes according to the chart above | ||
# Step 2 | # Step 2: Add 50μL of DNA sample mix to the corresponding reaction tube (Note: use each disposable pipette tip only once to avoid cross-contamination) | ||
# Step 3 | # Step 3: Place to reaction tubes into the thermocycler and run the above program | ||
'''PCR Reaction Mix''' | '''PCR Reaction Mix''' | ||
The PCR reaction mix will contain 8 tubes. Each tube will contain 50μL of the following: | |||
*Taq DNA polymerase | |||
*MgCl2 | |||
*dNTP's | |||
Revision as of 13:22, 23 October 2013
BME 100 Fall 2013 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | ||||||||||||
OUR TEAMLAB 1 WRITE-UPInitial Machine TestingThe Original Design Experimenting With the Connections When we unplugged (part 3) from (part 6), the machine ... (did what? fill in your answer) The LCD screen turned off. When we unplugged the white wire that connects (part 6) to (part 2), the machine ... (did what? fill in your answer) The temperature changed from 26.7 celsius degree to -40 celsius degree. Test Run (Write the date you first tested Open PCR and your experience(s) with the machine)
ProtocolsThermal Cycler Program
Research and DevelopmentPCR - The Underlying Technology (Add a write-up, essay-style, organized into paragrpahs with descriptive headers, based on the Q&A's from Section three of your worksheet) (BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the PCR video/ tutorial might be useful. Be sure to credit the sources if you borrow images.)
|