Difference between revisions of "Antarctic Phosphatase"

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==Notes==
 
==Notes==
  
This enzyme is preferable to either [http://www.neb.com/nebecomm/products/productM0290.asp Calf Intestinal Phosphatase] or Shrimp Alkaline Phosphatase because it is more easily heat-inactivated.
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This enzyme is preferable to either [http://www.neb.com/nebecomm/products/productM0290.asp Calf Intestinal Phosphatase] or Shrimp Alkaline Phosphatase because it is more easily heat-inactivated.  After phosphatase treatment of a vector, any remaining enzymatic activity would remove 5' phosphates from the insert in a subsequent ligation reaction, preventing a successful reaction.  Thus, it is critical that the phosphatase be inactivated, purified away, or digested with Proteinase K.
  
 
[[Category:Material]] [[Category:Enzyme]] [[Category:Phosphatase]]
 
[[Category:Material]] [[Category:Enzyme]] [[Category:Phosphatase]]

Latest revision as of 18:34, 18 August 2011

Website

NEB's Antarctic Phosphatase

Associated protocols

Phosphatase treatment of linearized vector

Notes

This enzyme is preferable to either Calf Intestinal Phosphatase or Shrimp Alkaline Phosphatase because it is more easily heat-inactivated. After phosphatase treatment of a vector, any remaining enzymatic activity would remove 5' phosphates from the insert in a subsequent ligation reaction, preventing a successful reaction. Thus, it is critical that the phosphatase be inactivated, purified away, or digested with Proteinase K.