From OpenWetWare
Revision as of 19:10, 18 October 2012 by Austin W. Jones (talk | contribs) (Digestion Enzyme Test)
Jump to: navigation, search
Owwnotebook icon.png Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Digestion Enzyme Test

  • Tested the digestion of Xba1 and Spe1 enzymes by incubating pSB1A7 plasmid in each enzyme for 10, 20, and 30 min. Results were checked on a gel, compared to undigested plasmid.


Samples in lanes 3, 4, and 5 were incubated with Xba1 for 10, 20, and 30min, respectively. Lane 6 contained undigested plasmid. Samples in lanes 7, 8, and 9 were incubated with Spe1 for 10, 20, and 30min, respectively. The gel shows good digestion by both enzymes at all time increments, as compared to circular plasmid, which moved a significantly shorter distance (Lane 6).