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Digestion Enzyme Test

  • Tested the digestion of Xba1 and Spe1 enzymes by incubating pSB1A7 plasmid in each enzyme for 10, 20, and 30 min. Results were checked on a gel, compared to undigested plasmid.


Samples in lanes 3, 4, and 5 were incubated with Xba1 for 10, 20, and 30min, respectively. Lane 6 contained undigested plasmid. Samples in lanes 7, 8, and 9 were incubated with Spe1 for 10, 20, and 30min, respectively. The gel shows good digestion by both enzymes at all time increments, as compared to circular plasmid, which moved a significantly shorter distance (Lane 6).