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PCR DNA Gel Extraction and Purification and the Beginning of T Vector Ligation
On Tuesday we ran a gel electrophoresis check on our PCR and it yielded these results:
Also note the functional positive control in lane 10
Based on these results, we believe we have amplified our target gene (pigI) so we extracted the DNA from the gel and purified it with a GeneJet Gel Extraction kit (Lot 00040498). We ran a gel electrophoresis check on the sample obtained from gel extraction and it yielded these results:
This gel confirms our belief that we have our target gene, so we proceeded to the next step which is transformation and cloning of our DNA using a T-vector. To this end, we started the T Vector Ligation, using the Promega pGEM-T Easy Vector Ligation "Cloning PCR" protocol and will begin transformation on next Tuesday.