Difference between revisions of "840:153g:Projects/project22/2012/09/27"

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(Gel Electrophoresis Results and PCR Set Up)
(Gel Electrophoresis Results and PCR Set Up)
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==Gel Electrophoresis Results and PCR Set Up==
 
==Gel Electrophoresis Results and PCR Set Up==
* DNA was tested for using gel electrophoresis, 5 samples were tested, both digested and undigested for a total of 10 wells used. The results of the gel suggested that the DNA may have been diluted too far, and thus created very weak bands in the gel. Two bands were visible, in lanes 7 and 11. Lane 7 showed the best band, which was sample 2 that was digested. This sample was chosen for use in PCR, because it was digested and had a visible band, we knew that it was digestible. Next week, we will check the results of the PCR.
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* DNA was tested for using gel electrophoresis, 5 samples were tested, both digested and undigested for a total of 10 wells used. The results of the gel suggested that the DNA may have been diluted too far, and thus created very weak bands in the gel. Two bands were visible, in lanes 7 and 11. Lane 7 showed the best band, which was sample 3 that was digested. This sample was chosen for use in PCR, because it was digested and had a visible band, we knew that the DNA in the sample was functional. Next week, we will check the results of the PCR.
  
Picture of simple gel electrophoresis results [[Image:!G229251.2_(2).png]]
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Picture of simple gel electrophoresis results - Note the only relevant brightness appears in lanes 7 and 11
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[[Image:!G229251.2_(2).png]]
  
 
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Revision as of 15:54, 18 October 2012

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Gel Electrophoresis Results and PCR Set Up

  • DNA was tested for using gel electrophoresis, 5 samples were tested, both digested and undigested for a total of 10 wells used. The results of the gel suggested that the DNA may have been diluted too far, and thus created very weak bands in the gel. Two bands were visible, in lanes 7 and 11. Lane 7 showed the best band, which was sample 3 that was digested. This sample was chosen for use in PCR, because it was digested and had a visible band, we knew that the DNA in the sample was functional. Next week, we will check the results of the PCR.

Picture of simple gel electrophoresis results - Note the only relevant brightness appears in lanes 7 and 11 !G229251.2 (2).png