Difference between revisions of "840:153g:Projects/project16/2011/09/20"

From OpenWetWare
Jump to: navigation, search
(DNA Extraction using Chelex)
(DNA Extraction using Chelex)
Line 6: Line 6:
 
| colspan="2"|
 
| colspan="2"|
 
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
 
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==DNA Extraction using Chelex==
+
==Constructing and Running an Agarose Gel Electrophoresis==
Today we ran a Agarose gel electrophoresis to determine if we successfully extracted any DNA. We ran three different experiments, one lane contained the solunate with EcoRI restriction enzyme, another lane contained an undigested solunate, and the third lane contained the solunate with PstI restriction enzyme. Our results showed that we did have DNA but it wasn't purified enough for a PCR reaction. Therefore we decided to purify our DNA before we run a PCR reaction.
+
Today we ran a Agarose gel electrophoresis to determine if we successfully extracted any DNA. We ran three different experiments, one lane contained the aqueous DNA with EcoRI restriction enzyme, another lane contained an undigested DNA, and the third lane contained the DNA with PstI restriction enzyme. Our results showed that we did have DNA but it wasn't pure enough for a PCR reaction. Therefore we decided to purify our DNA before we run a PCR reaction.
  
 
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
 
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Revision as of 15:28, 22 September 2011

Owwnotebook icon.png Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Constructing and Running an Agarose Gel Electrophoresis

Today we ran a Agarose gel electrophoresis to determine if we successfully extracted any DNA. We ran three different experiments, one lane contained the aqueous DNA with EcoRI restriction enzyme, another lane contained an undigested DNA, and the third lane contained the DNA with PstI restriction enzyme. Our results showed that we did have DNA but it wasn't pure enough for a PCR reaction. Therefore we decided to purify our DNA before we run a PCR reaction.