Difference between revisions of "7-AAD"

From OpenWetWare
Jump to: navigation, search
Line 12: Line 12:
 
* 546/647
 
* 546/647
 
* Nucleic acid dye
 
* Nucleic acid dye
 +
* Often used in conjunction with [[Annexin-V]] when assaying for apoptotic cells
 
* "The '''advantage of 7-AAD''' over PI is the ability to be used '''in conjunction with''' phycoerythrin ('''PE''')- '''and''' fluorescein isothiocyanate ('''FITC''')-labeled monoclonal antibodies in 2-color analysis, with minimal spectral overlap between 7-AAD, PE and FITC fluorescence emissions. The 7-AAD fluorescence is detected in the '''far red range''' of the spectrum (650 nm long-pass filter).
 
* "The '''advantage of 7-AAD''' over PI is the ability to be used '''in conjunction with''' phycoerythrin ('''PE''')- '''and''' fluorescein isothiocyanate ('''FITC''')-labeled monoclonal antibodies in 2-color analysis, with minimal spectral overlap between 7-AAD, PE and FITC fluorescence emissions. The 7-AAD fluorescence is detected in the '''far red range''' of the spectrum (650 nm long-pass filter).
  

Revision as of 00:49, 8 July 2008

7-aminoactinomycin-D

Purpose

  • Staining dead cells

Procurement

Use

  • 546/647
  • Nucleic acid dye
  • Often used in conjunction with Annexin-V when assaying for apoptotic cells
  • "The advantage of 7-AAD over PI is the ability to be used in conjunction with phycoerythrin (PE)- and fluorescein isothiocyanate (FITC)-labeled monoclonal antibodies in 2-color analysis, with minimal spectral overlap between 7-AAD, PE and FITC fluorescence emissions. The 7-AAD fluorescence is detected in the far red range of the spectrum (650 nm long-pass filter).

Safety

References

  • MC O’Brien, et al. (1995) Cytometry 19:243
  • I Schmid et al. (1992) Cytometry 13:204