Swartz:Protocols

   

Research

Papers

People

Contact

Getting settled in the Swartz Lab

Protocols

Cloning

• Swartz Lab Molecular Biology Handbook
• Restriction Enzyme Library

DNA Design

• Primer Design

Gene Synthesis

• PCR
• Gene Synthesis
• Restriction Enzyme Digestion/Ligations
• Quikchange
• [| Gel Purification]
• Column Regeneration

Expression Templates

Plasmids

• OWW Plasmid Miniprep or | Swartz Lab Version
• Plasmid Maxiprep

Organisms

• E.coli Transformation

• Other Cloning Protocols

Cell Free Protein Synthesis

• Preparing linear DNA template for CFPS
• 1L Extract Prep
• Cell Free Protein Synthesis
• How to do an Autoradiogram
• Western Blotting
• High Density Fermentation

Protein Purification

• His-Tag Purification
• Ribosome purification

Assays

• Nanodrop
• Using the spec for DNA Quantification
• Sample prep for MS
• Spin assay for ribsome binding
• HPLC
• Cytochrome C
• Methyl Viologen
• H2 Production (GC)

Miscellaneous

• Anaerobic Protein Production
• Cell culture for A20 B-cells

Solutions

• Buffer Recipes