Smolke:Protocols/General Maintenance

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Cryo container Fill the container after every opening. LN2 should be above the platform but below the lowest box (i.e., samples should be in the vapor phase). CO2 tanks All TC room users must know how to read the gauge and change and vent the cylinders. New cylinders are sometimes left in the building's cylinder cage, but usually delivered to lab. The empty cylinder must be disconnected before the new cylinder is delivered. Biohazard waste Waste is picked up every other Thursday. The receipt should be placed in the invoice drawer. Replacement bags for biohazard waste are in the cabinet under the radioactivity bench. Trash Regular trash should be taken out of the TC room each night for pickup from the janitorial service. Water bath Leo will empty and refill every month. Make sure that media and other reagents are not left in the water bath. Incubators The water jackets on the incubators must be filled with DI water. DI water is purchased for the incubators. The funnel for filling the jackets are in the cabinet below the TC bench. The water pans in the incubator must be routinely cleaned. To do so water should be changed and fresh fungicide should be added. Equipment All TC room equipment (e.g., tube racks, timers, EtOH bottles, gloves) should stay in the TC room. Also, outside equipment should not be brought in to the TC room. The tabletop centrifuge (on the radioactive station) should remain closed when not in use. The tacky mat should be changed as necessary. Spare mats are on the shelf above the radioactive station and must be trimmed to the right size. General safety Remember to turn off the gas valve when using the burners inside the hoods. Biohazard waste should be as dry as possible before putting in the container. Waste container lid should be closed immediately after use.