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Project name
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3/25/13
- Prepped plasmids of KAH201 and determined concentration and purity.
Sample |
260/280 |
ng/μL
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1. KAH201 (Plasmid) |
1.795 |
54.054
|
2. KAH201 (Plasmid) |
1.708 |
47.244
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Reagent |
Volume |
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30 μL/lane, 1% agarose; Ladder
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DNA (plasmid) |
25 μL
|
10x buffer |
3.0
|
enzyme 1 (Pst1) |
1.0
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enzyme 2 (Xba1 or Spe1) |
1.0
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dH2O |
0.0 μL
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30 μL --> 37°C/ ~15 min.
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> Measure conc.'s of gel purified fragments
Sample |
260/280 |
ng/μL
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1. KAH201 (S/P) |
0.727 |
2.54
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2. KAH182 (X/P) |
0.789 |
13596
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