User:Karmella Haynes/Notebook/BioBrick cloning/2015/04/14: Difference between revisions
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|- valign="top" | |- valign="top" | ||
| bgcolor=#cfcfcf | Reagent | | bgcolor=#cfcfcf | Reagent | ||
| bgcolor=#cfcfcf | Rxn1 | | bgcolor=#cfcfcf | Rxn1,2 | ||
| bgcolor=#cfcfcf | | | bgcolor=#cfcfcf | Rxn3,4 | ||
| rowspan="7" | Expected:<br>1,2. Gal4DB-mCh = 1170<br>3,4. ATF2 = 906<br> | | rowspan="7" | Expected:<br>1,2. Gal4DB-mCh = 1170<br>3,4. ATF2 = 906<br> | ||
| rowspan="7" | [[Image:somegel.jpg|200px|Hover name]]<br>10 μL/lane, 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | | rowspan="7" | [[Image:somegel.jpg|200px|Hover name]]<br>10 μL/lane, 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | ||
|- | |- | ||
| Template || 2.0 | | Template || 0.5 || 2.0 | ||
|- | |- | ||
| 10 uM fwd primer || 1.0 | | 10 uM fwd primer || 1.0 || 1.0 | ||
|- | |- | ||
| 10 uM rev primer || 1.0 | | 10 uM rev primer || 1.0 || 1.0 | ||
|- | |- | ||
| 10 mM dNTPs || 1.0 | | 10 mM dNTPs || 1.0 || 1.0 | ||
|- | |- | ||
| Phusion pol. || 0.5 | | Phusion pol. || 0.5 || 0.5 | ||
|- | |- | ||
| 5x GC buffer || 5.0 | | 5x GC buffer || 5.0 || 5.0 | ||
|- | |- | ||
| dH<sub>2</sub>O || 39.5 | | dH<sub>2</sub>O || 41.0 || 39.5 | ||
|- | |- | ||
| || 50.0 | | || 50.0 || 50.0 | ||
|} | |} | ||
Revision as of 16:19, 14 April 2015
Karmella's BioBrick Cloning | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
04/14/15
LCR Protocol Development
Program: Phusion (block A) - edited to include 65°C - 55°C annealing gradient
Conclusion
Gal4DB-mCh - plasmid
Program: Phusion (block B)
Conclusion
Program: Phusion (block A) - edited to include 65°C - 55°C annealing gradient
Conclusion
Program: Phusion (block B)
Conclusion
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