Gill:10 X M9 salts + glucose: Difference between revisions
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New page: ==Materials== 1000 mL: *dH<sub>2</sub>O 700 ml *Na<sub>2</sub>HPO<sub>4</sub> • 7H<sub>2</sub>O 128 g *KH<sub>2</sub>PO<sub>4</sub> 30 g *NaCl 5 g *NH<sub>4</sub>Cl 10 g *Glucose 40 g ... |
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| Line 3: | Line 3: | ||
1000 mL: | 1000 mL: | ||
*dH<sub>2</sub>O | *700 ml dH<sub>2</sub>O | ||
*Na<sub>2</sub>HPO<sub>4</sub> • 7H<sub>2</sub>O | *128 g Na<sub>2</sub>HPO<sub>4</sub> • 7H<sub>2</sub>O | ||
*KH<sub>2</sub>PO<sub>4</sub> | *30 g KH<sub>2</sub>PO<sub>4</sub> | ||
* | *5 g NaCl | ||
*NH<sub>4</sub>Cl | *10 g NH<sub>4</sub>Cl | ||
* | *40 g Glucose | ||
==Procedure== | ==Procedure== | ||
Latest revision as of 21:11, 23 April 2013
Materials
1000 mL:
- 700 ml dH2O
- 128 g Na2HPO4 • 7H2O
- 30 g KH2PO4
- 5 g NaCl
- 10 g NH4Cl
- 40 g Glucose
Procedure
Add all components to water, dissolve, adjust to 1000 ml. Filter sterilize at 0.22 µm (do not autoclave), store at RT. If using anhydrous Na2HPO4, multiply the weight for this component by 0.53.
Notes
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
