User:NYOUSHA Yousefi/Notebook/Protein Engineering/2013/01/29: Difference between revisions
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# 5ul of Ampicillin was added to both test tubes | # 5ul of Ampicillin was added to both test tubes | ||
# The frozen cells of PQE-80 bacteria in glycerol were taken out of the -80 degree C fridge | # The frozen cells of PQE-80 bacteria in glycerol were taken out of the -80 degree C fridge | ||
# Some PQE-80 bacteria cells were added to these LB | # Some PQE-80 bacteria cells were added to these LB mediums under sterile conditions | ||
# PQE-80 bacteria were also added to four erlyn meyer flasks containing LB media and 35ul Ampicillin | # PQE-80 bacteria were also added to four erlyn meyer flasks containing LB media and 35ul Ampicillin | ||
# The tubes and flasks were placed in the incubator/shaker at 37 degree C for 24h | # The tubes and flasks were placed in the incubator/shaker at 37 degree C for 24h |
Revision as of 06:59, 5 February 2013
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Entry titlePreparing protein solutions and lyophilization Objective 1Eight different protein solutions were prepared for lyophilization Procedure 1
Objective 2Culturing PQE-80 antibiotic resistant bacteria Procedure 2
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