IGEM:IMPERIAL/2006/project/parts/lab status

Lab Status

This page is for us to list the stage that needed parts are at in the lab. If anyone wants a part cultured that is not listed here, please add it to the bottom of the table.

Status Hierarchy

• Not Cultured
  • Part is still sitting in iGEM DNA Plate
• Cultured
  • Part has been transformed into E.coli and has been cultured up
• Mini-preped
  • DNA has been mini-preped and assessed by restriction digest
• Maxi-preped
  • DNA has been maxi-preped in preperation for a ligation
• Ligated
  • DNA has been successfully ligated
  • Please indicate the parts ligated together in the 'Part' column of the table
• Assayed (I ran out of colours, okay? - TomH)
  • The construct has been successfully assayed
    

Current Part Status

Part	Well	Plate	Status	Remarks	Test Part	Final Part
F2620	6B	1	Maxi-preped	Ligated to C0261+I13504	J37015	Prey
T9002	19B	2	Mini-preped	Testing phase underway	Itself	Not in final
C0261	24A	1	Maxi-preped	To be ligated to I13504	J37015	Prey
R0062	9G	1	Maxi-preped	Too small to miniprep	J37019	Predator
B0034	3O	1	Maxi-preped	To be ligated to C0062	J37022	Predator, AiiA const
C0062	7A	1	Maxi-preped	To be ligated to B0034	J37019	Predator
I13504	12D	1	Maxi-preped	To be ligated to C0261 and I13033 separately	J37015, J37016	Not in final
I13033	2H	1	Maxi-preped	To be ligated to I13504, Successful 2nd attempt (mini-prep).	J37020	Not in final
J04500	16P	1	Maxi-preped	Ligated to J37024	J37022	Not in final, Cre
C0060	5M	1	Maxi-preped		J37022	AiiA const
B0015	1I	1	Maxi-preped		J37022	AiiA const, Cre
I13207	2P	1	Mini-preped	L-Arabinose located, ready for testing	Itself	Not in final
I13521	18I	1	Maxi-preped	PCR being used to attach LoxP site	J37015Cre	Prey
B0021	1M	1	Maxi-preped	PCR being used to attach LoxP site	J37015Cre	Prey
S01656	4G	2	Mini-preped	Testing Phase Underway, Kanamycin resistant	Itself	Not in final

Ligation Process

Part	Status	Remarks	Test Part	Final Part
J37015 intermediate	Maxi-preped	C0261(24A) + I13504(12D)	J37015	Prey
J37015	Maxi-preped, Sequenced	F2620(6B) + (J37015 intermediate)	Itself	Prey
RS Intermediate	Maxi-preped	Riboswitch + (C0261(24A)+I13504(12D))	J37015RS	Prey
J37015RS	Maxi-preped, sent for sequencing	F2620(6B) + (RS Intermediate)	Itself	Prey
J37033	Maxi-preped Batch 1	B0034(30) + C0062(7A)	J37019	Predator test construct
J37019	Maxi-preped, Sequenced	R0062(9G) + J37033	J37016	Predator test constructs, Predator
J37016	Maxi-preped, Sequenced	J37019 + I13504(12D)	Itself	Predator test construct
J37031	Maxi-preped	J37019 + B0015(1I)	J37020	Predator test construct
J37032	Maxi-preped	R0062(9G) + I13504 (12D)	J37020	Predator test construct
J37020	Maxi-preped, sent for sequencing	J37031 + J37032	Itself	Predator test construct
J37023	Maxi-preped, sent for sequencing	AiiA (C0060)w/FLAG & LVA	J37024	Predator, aiiA test construct
J37024	Being re-ligated	J37023 + B0015(1I)	J37022, J37025	Predator, aiiA test construct
J37022	Waiting for J37024	J04500 (16P) + J37024	Itself	aiiA test construct
J37025	Waiting for J37024	B0034(30) + J37024	Predator	Predator
J37018	'	J37031+R0062		Predator
12D->2H	Troubleshooting	Problem with 2H - abandoned	J37020	Predator

Parts No Longer Needed

Part	Well	Plate	Status	Remarks
E0040	5H	1	Mini-preped	No longer needed
I13272	4F	1	Mini-preped	No longer needed
F2621	4N	1	Mini-preped	No longer needed
I13273	4H	1	Mini-preped	No longer needed
I13208	4J	1	Mini-preped	No longer needed
I0460	11I	1	Cultured	No longer needed
F1610	1B	2	Mini-preped	No longer needed
I0403	5P	1	Mini-preped	No longer needed
E0434	20J	1	Mini-preped	No longer needed
I14032	17K	2	Cultured	No longer needed
C0260	24C	1	Mini-preped	No longer needed
C0160	9F	1	Cultured	No longer needed