User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/11/03: Difference between revisions
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1L of sterile water | 1L of sterile water | ||
* 2 of 1L LB media and 2 of 35mL LB media were placed in autoclave. The autoclave goes through the following procedure for liquid cycles: | * 2 of 1L LB media and 2 of 35mL LB media were placed in autoclave. The autoclave goes through the following procedure for liquid cycles: | ||
** Water was filled to bottom of autoclave | |||
** Knob of autoclave was turned to STE | |||
** 2 of 1L LB media and 2 of 35mL of LB media were loaded into autoclave | |||
** Door was closed tightly | |||
** Timer was set to 60 minutes | |||
** After 60 minutes, the knob was left on STE for 30 minutes and until the pressure needle turned to 0 | |||
** Door was opened and autoclaved LB media was unloaded | |||
* The above procedure was repeated twice | * The above procedure was repeated twice | ||
* Oribital shaker was prewarmed to 37°C | * Oribital shaker was prewarmed to 37°C |
Revision as of 20:43, 24 November 2012
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Purpose
Procedure
0.88mg of LB broth media powder 35mL of sterile water
25g of LB broth media powder 1L of sterile water
Notes
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