User:Sean P Corum/Notebook/PHIX174 Cell Free/2012/07/11: Difference between revisions
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===Characterization B: Expression of PHIX174 promoters fused to UTR1-deGFP.=== | ===Characterization B: Expression of PHIX174 promoters fused to UTR1-deGFP.=== | ||
* Performed ligation of latest batch of SphI-PX-NheI linkers and pBEST-SphI//NheI-UTR1-deGFP-T500 backbone to obtain the following products: | * Performed [http://openwetware.org/wiki/Corum:T4_Ligation ligation] of latest batch of SphI-PX-NheI linkers and pBEST-SphI//NheI-UTR1-deGFP-T500 backbone to obtain the following products: | ||
*# pBEST-PB-UTR1-deGFP-T500 | *# pBEST-PB-UTR1-deGFP-T500 | ||
*# pBEST-PD-UTR1-deGFP-T500 | *# pBEST-PD-UTR1-deGFP-T500 |
Revision as of 18:25, 10 July 2012
PHIX174 Cell Free Expression | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Characterization B: Expression of PHIX174 promoters fused to UTR1-deGFP.
Characterization C: Expression of PHIX174 promoters fused to UTRX-deGFP.
Hypothesis 2: Gene L is necessary for phage propagation.
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