Characterization B: Expression of PHIX174 promoters fused to UTR1-deGFP.
- Performed transformation of yesterday's ligation products into JM109 competent cells to obtain:
- pBEST-PB-UTR1-deGFP-T500
- pBEST-PD-UTR1-deGFP-T500
- pBEST-PF-UTR1-deGFP-T500
- pBEST-PG-UTR1-deGFP-T500
- pBEST-PL-UTR1-deGFP-T500
- pBEST-PL-L-PA-UTR1-deGFP-T500
- -linker control
Characterization C: Expression of PHIX174 promoters fused to UTRX-deGFP.
- Performed transformation of yesterday's ligation products into JM109 competent cells to obtain:
- pBEST-PB-UTRB-deGFP-T500
- pBEST-PD-UTRD-deGFP-T500
- pBEST-PF-UTRF-deGFP-T500
- pBEST-PG-UTRG-deGFP-T500
- pBEST-PL-UTRL-deGFP-T500
- -linker control
Hypothesis 2: Gene L is necessary for phage propagation.
- On hold until new primers arrive.
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