Biomod/2011/TUM/TNT/Glossary: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
← Older editNewer edit →
No edit summary
No edit summary
Line 450: Line 450:
<h3>FOB</h3>FOB is short for folding buffer. It contains 50mM Tris and 10mM EDTA. FOBxM refers to FOB without MgCl<sub>2</sub>, whereas FOB20 is FOB with 20mM MgCl<sub>2</sub>
<h3>FOB</h3>FOB is short for folding buffer. It contains 50mM Tris and 10mM EDTA. FOBxM refers to FOB without MgCl<sub>2</sub>, whereas FOB20 is FOB with 20mM MgCl<sub>2</sub>


 
<h3>FRET</h3>
In short, FRET is a fluorescence based approach often used in biosciences to measure distances in the lengthscale of up to ca. 10nm. For more explanation, please read the [http://openwetware.org/wiki/Biomod/2011/TUM/TNT/Methods/FRET entry in the methods section].


<h3>Intercalation</h3>Intercalation is known to occur without interfering with hydrogen bonding of the base pairs and obeys the nearest neighbor exclusion principle. When DNA is saturated with intercalators, every second potential intercalation site on the helix remains empty. In order for intercalation to ensue, theDNAbase pairs separate 3.4 °A to form a cavity for the incoming planar ligand through localized left-handed unwinding of the duplex. The normal twist of B-formDNA is 36◦ (10 base pairs per one turn of 360◦). In order to accommodate the ligand, a reduction of this rotation occurs. The unwinding angle varies with the geometry of the ligand–DNA complex.
<h3>Intercalation</h3>Intercalation is known to occur without interfering with hydrogen bonding of the base pairs and obeys the nearest neighbor exclusion principle. When DNA is saturated with intercalators, every second potential intercalation site on the helix remains empty. In order for intercalation to ensue, theDNAbase pairs separate 3.4 °A to form a cavity for the incoming planar ligand through localized left-handed unwinding of the duplex. The normal twist of B-formDNA is 36◦ (10 base pairs per one turn of 360◦). In order to accommodate the ligand, a reduction of this rotation occurs. The unwinding angle varies with the geometry of the ligand–DNA complex.

Revision as of 22:17, 31 October 2011

<html> 

  <style>
  1. column-one { display:none; width:0px;}

.container{background-color: #f5f5f5; margin-top:50px} .OWWNBcpCurrentDateFilled {display: none;}

  1. content { width: 0px; margin: 0 auto auto 0; padding: 1em 1em 1em 1em; align: center;}
  1. column-content {width: 0px; float: left; margin: 0 0 0 0;padding: 0;}

.firstHeading {display:none; width:0px;}

  1. globalWrapper{ width:1280px;}

h6 { color: black; background: none; font-weight: normal; margin: 0; padding-top: .5em; padding-bottom: .17em; border-bottom: 0px solid #aaa; } h6 { font-size: 188%; } h6 .editsection { font-size: 53%; }

  1. column-one {display:none; width:0px;background-color: #ffffff;}
  1. content{ margin: 0 0 0 0; padding: 1em 1em 1em 1em; position: center; width: 800px;background-color: #f5f5f5; }

.container{ width: 800px; margin: auto; background-color: #ffffff; text-align:justify; font-family: helvetica, arial, sans-serif; color:#555555; margin-top:25px; }

  1. bodyContent{ width: 1267px; align: center; background-color: #ffffff;}
  1. column-content{width: 1280px;background-color: #f5f5f5;}

.firstHeading { display:none;width:0px;background-color: #ffffff;}

  1. header{position: center; width: 800px;background-color: #ffffff;}
  1. footer{position: center;}


  1. bodyContent h6 {

margin-bottom: .6em; }

  1. bodyContent .plainlinks a {

background: none !important; padding: 0 !important; } .portlet h6 { margin: 0; padding: 0; }

<style type="text/css"> body { 

   font-family: helvetica, arial, sans-serif;
   color: black;

background:#f5f5f5; 

 }

ul#topnav {

margin: 0 0 0 0px ; padding: 0 0 0 3px; float: left; width: 800px; list-style: none; position: relative; 

       font-family: helvetica, arial, sans-serif;

font-size: 12px; background: #f5f5f5; 

       height: 5px;

} ul#topnav li { float: left; margin: 0; padding: 0; border-right: 1px solid #ffffff; /*--Divider for each parent level links--*/ } ul#topnav li a { padding: 11px 15px; display: block; color: #333333; text-decoration: none; } ul#topnav li:hover { background: #cccccc;} /*--Notice the hover color is on the list item itself, not on the link. This is so it can stay highlighted even when hovering over the subnav--*/

ul#topnav li span { float: left; padding: 15px 0; position: absolute; left: 0; top:30px; display: inline; /*--Hide by default--*/ width: 800px; background: #cccccc; color: #333333; /*--Bottom right rounded corner--*/ -moz-border-radius-bottomright: 5px; -khtml-border-radius-bottomright: 5px; -webkit-border-bottom-right-radius: 5px; /*--Bottom left rounded corner--*/ -moz-border-radius-bottomleft: 5px; -khtml-border-radius-bottomleft: 5px; -webkit-border-bottom-left-radius: 5px; } ul#topnav li:hover span { display: block; } /*--Show subnav on hover--*/ ul#topnav li span a { display: inline;} /*--Since we declared a link style on the parent list link, we will correct it back to its original state--*/ ul#topnav li span a:hover {text-decoration: underline;}


  1. abstractandlinks{

display:block; width:800px; background-color:#f5f5f5; text-align:justify;font-family: helvetica, arial, sans-serif; color:#555555; margin-top:0px; }

  1. innerBox

{ display: block; padding: 5px; }

  1. abstract{

font-family: helvetica, arial, sans-serif; width: 490px; margin-top: 20px; padding:0px 5px; float: left;background-color:#f5f5f5; ; }

  1. links{

font-family: helvetica, arial, sans-serif; width: 290px; margin-top: 20px; padding:0px 5px; float: left; background-color:#f5f5f5;; } .clear { clear:both; }

  1. tour{

font-family: helvetica, arial, sans-serif; width: 790px; padding:0px 5px; margin-top: 1em, margin-bottom: 1em; float: left;background:#f5f5f5; font-size: 2em; }

  1. struktur{

font-family: helvetica, arial, sans-serif; width: 790px; padding:0px 5px; float: left;background:#f5f5f5; }

  1. toctitle {align: left; text-align: left; }
  2. table.toc {background-color:"#5f5f5f";align: left; text-align: left;}
  3. toc #toctitle, .toc #toctitle, #toc .toctitle, .toc .toctitle {

text-align: left; 

}
  1. toc, .toc, .mw-warning {

background-color: #f5f5f5; border-bottom-color: rgb(170, 170, 170); border-bottom-style: solid; border-bottom-width: 0px; border-left-color: rgb(170, 170, 170); border-left-style: solid; border-left-width: 0px; border-right-color: rgb(170, 170, 170); border-right-style: solid; border-right-width: 0px; border-top-color: rgb(170, 170, 170); border-top-style: solid; border-top-width: 0px; font-size: 105%; padding-bottom: 5px; padding-left: 5px; padding-right: 5px; padding-top: 5px; align: left; text-align: left; display: left 

}


</style>

<head>

<script type="text/javascript"> var fullDates = new Array('08/11/2011','08/12/2011','08/16/2011','08/17/2011','08/23/2011','08/24/2011','08/25/2011','08/29/2011','08/30/2011','08/31/2011','09/01/2011','09/02/2011','09/06/2011','09/12/2011','09/13/2011','09/14/2011','09/15/2011','09/16/2011','09/19/2011','09/20/2011','09/21/2011','09/27/2011','09/28/2011','09/29/2011','09/30/2011','10/05/2011','10/06/2011','10/07/2011','10/10/2011','10/12/2011','10/13/2011','10/14/2011','10/17/2011','10/18/2011','10/19/2011','10/20/2011','10/21/2011','10/26/2011','10/27/2011','10/28/2011','10/30/2011');

$(document).ready(function() {

$("ul#topnav li").hover(function() { //Hover over event on list item $(this).css({ 'background' : '#cccccc'}); //Add background color and image on hovered list item $(this).find("span").show(); //Show the subnav

} ,

$("ul#topnav li").oneclick(function() { //Hover over event on list item $(this).css({ 'background' : '#cccccc'; 'display' : 'inline'}); //Add background color and image on hovered list item $(this).find("span").show(); //Show the subnav

} ,

function() { //on hover out...


$(this).css({ 'background' : 'none'}); //Ditch the background

//$(this).find("span").hide(); //Hide the subnav });

}); </script>


<script type="text/javascript" src="http://ajax.googleapis.com/ajax/libs/jquery/1.3/jquery.min.js"></script>


<meta http-equiv="Content-Type" content="text/html; charset=utf-8" /> 

 <script type="text/javascript" src="http://ajax.googleapis.com/ajax/libs/jquery/1.4.2/jquery.min.js"></script>
 <script type="text/javascript">
  $(document).ready(function(){
   $('#lside1').mouseover(function() {
    $('#lside1').stop().animate({backgroundPosition: '0px 0px'}, 1000);
   });
   $('#lside1').mouseleave(function() {
    $('#lside1').stop().animate({backgroundPosition: '-250px 0px'}, 1000);
   });
   $('#lside2').mouseover(function() {
    $('#lside2').stop().animate({backgroundPosition: '0px 0px'}, 1000);
   });
   $('#lside2').mouseleave(function() {
    $('#lside2').stop().animate({backgroundPosition: '-250px 0px'}, 1000);
   });
   $('#lside3').mouseover(function() {
    $('#lside3').stop().animate({backgroundPosition: '0px 0px'}, 1000);
   });
   $('#lside3').mouseleave(function() {
    $('#lside3').stop().animate({backgroundPosition: '-250px 0px'}, 1000);
   });
  });
 </script>

<style type="text/css"> 

  #side1 {
   width: 250px;
   height: 100px;
   position: relative;
   top: 0px;
   left: 20px;}

  #lside1 {
   width: 250px;
   height: 100px;
   background: url(http://www.tftshop.net/Testbild_100ppi.jpg) -250px 0px no-repeat;
   position: absolute;
   top: 0px;
   left: 0px;}

  #side2 {
   width: 250px;
   height: 100px;
   position: relative;
   top: 0px;
   left: 20px;}

  #lside2 {
   width: 250px;
   height: 100px;
   background: url(http://www.tftshop.net/Testbild_100ppi.jpg) -250px 0px no-repeat;
   position: absolute;
   top: 0px;
   left: 0px;}

  #side3 {
   width: 250px;
   height: 100px;
   position: relative;
   top: 0px;
   left: 20px;}

  #lside3 {
   width: 250px;
   height: 100px;
   background: url(http://www.tftshop.net/Testbild_100ppi.jpg) -250px 0px no-repeat;
   position: absolute;
   top: 0px;
   left: 0px;}

 </style>

<script type="text/javascript" src="http://ajax.googleapis.com/ajax/libs/jquery/1.4.2/jquery.min.js"></script> <script type="text/javascript"> 

$(document).ready(function(){
 $('#mainpic').attr('src',

'http://openwetware.org/wiki/Image:U1.png'); 

 $('#mainpic').attr('src',

'http://australian-kanguru-watchers.de/wp-content/uploads/2009/11/dnet24-testbild.jpg'); 

 $('#mainpic').attr('src',

'http://eschbaer.de/wp-content/uploads/final_testbild.png'); 

 $('#mainpic').attr('src',

'http://www.media-digest.com/wp-content/uploads/2009/05/test-pattern-briefs.jpg'); 

 $("#but1").mouseenter(function() {
  $('#mainpic').attr('src', 'http://www.cnet.co.uk/i/c/rv/e/digitalcameras/fujifilm/finepix_f200exr/f200exrstorygraphic1large.jpg');
 });
 $("#but2").mouseenter(function() {
  $('#mainpic').attr('src',

'http://xmas.tfoe.net/img/testbild.jpg'); 

 });
 $("#but3").mouseenter(function() {
  $('#mainpic').attr('src',

'http://www.tv-testbild.com/bilder/nbc-320.jpg'); 

 });
 $("#but4").mouseenter(function() {
  $('#mainpic').attr('src',

'http://www.tv-testbild.com/pictures/orf2.jpg'); 

 });

});

</script> <style type="text/css"> 

#maincon {
 width:800px;
 position:relative;}
#mainpic {
 width:800px;}
#but1 {
 width:60px;
 height:60px;
 position:absolute;
 left:88px;
 top:19px;
 z-index:2;
 background-image:url('http://openwetware.org/wiki/Image:U1.png');}
#but2 {
 width:60px;
 height:60px;
 position:absolute;
 left:489px;
 top:220px;
 z-index:2;
 background-image:url('http://upload.wikimedia.org/wikipedia/commons/thumb/0/07/Button_Icon_Red.svg/300px-Button_Icon_Red.svg.png');}
#but3 {
 width:60px;
 height:60px;
 position:absolute;
 left:320px;
 top:302px;
 z-index:2;
 background-image:url('http://www.lefinnois.net/aqua/aqua1.jpg');}
#but4 {
 width:60px;
 height:60px;
 position:absolute;
 left:509px;
 top:4px;
 z-index:2;
 background-image:url('http://www.lefinnois.net/aqua/aqua1.jpg');}

</style>



<title> 

       TUM NanU - Home

</title> </head>

<body background-color="#F0F0F0">




</body> </html>

Retrieved from "https://openwetware.org/mediawiki/index.php?title=Biomod/2011/TUM/TNT/Glossary&oldid=557339"