Referring to the relevance of this project accordingly the future goal should be to find a way to characterize DNA binding molecules. In the field of bioscience the Ramachandran plot is a nice way to show typical secondary structures of proteins.
In this case we would plot twist against length (fig 1). To do this it would be necessary to test more DNA binding molecules and to design a structure which is more sensitive to measure changes in length. This should be done based on the theoretical background which considers geometrical thoughts and information we gained during this project.
fig. 1: Classifying DNA-binders by twist and length change
By optimizing the structure based on our results it should be possible to create a device whose conformational changes can be precisely predetermined.
This would permit to use this structure the other way around. Knowing the outcome of conformational changes of DNA origami using a certain concentration of an well known DNA-binder will provide a valuable tool especial in the field of creating functional DNA origami.
Welche Experimente wären noch möglich/ sollten noch gemacht werden, um... zu zeigen. Ferne und nahe Zukunft. Was könnte damit noch untersucht werden (Sequenzspezifische Bindeproteine...)
- Struktur mit asymmetrischem feature um die richtung der projektion bestimmen zu können