Talk:20.109(S14):Complete Western and prepare damaged DNA (Day4): Difference between revisions
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===T/R=== | ===T/R=== | ||
[[Image:TR_M2D4_gel1_S14.jpg | left |thumb|275px|T/R Gel 1]] | |||
[[Image:M2D4-Gel2_Pnk-Grn-Blu.jpg | right |thumb|275px | T/R Gel 2]] | |||
<br style="clear:both;"/> | |||
===W/F=== | |||
Recall that lane 10 is singly digested (with ''EcoRV'') DNA prepared by Su for a size comparison to the full-length plasmid versus plasmid with no insert. | |||
[[Image:M2D4-Gel1_Pnk-Red-Blu-Su.jpg | left |thumb|325px | W/F Gel 1]] | |||
[[Image:M2D4-Gel2_Wht-Sil-Prp-Su.jpg | center |thumb|325px | W/F Gel 2]] | |||
[[Image:M2D4-Gel3_Grn-Ylw-Org-Su.jpg | left |thumb|325px | W/F Gel 3]] | |||
[[Image:M2D4-Gel2.2_Wht.jpg | center |thumb|325px | W/F Gel 2 extra run time]] | |||
<br style="clear:both;"/> | |||
==Westerns== | ==Westerns== | ||
===T/R=== | ===T/R=== | ||
[[Image:S14_M2D4_TR-Grn.JPG | left |thumb|250px | T/R WB, Green group]] | |||
[[Image:S14_M2D4_TR-Blu.JPG | right |thumb|250px | T/R WB, Blue group]] | |||
[[Image:S14_M2D4_TR-Ylw.JPG | center |thumb|250px | T/R WB, Yellow group]] | |||
[[Image:S14_M2D4_TR-pink.JPG | right |thumb|250px | T/R WB, pink group]] | |||
[[Image:S14_M2D4_TR-red.JPG | right |thumb|250px | T/R WB, red group]] | |||
[[Image:S14_M2D4_TR-orange.JPG | right |thumb|250px | T/R WB, orange group]] | |||
<br style="clear:both;"/> | |||
===W/F=== | ===W/F=== | ||
Hi folks -- I think these got flipped because they were sent from my phone. Please turn them in the usual orientation (and crop) for use in your reports. | |||
[[Image:S14_M2D4_WF-Ylw.JPG | right |thumb|250px | W/F Gel, Yellow group]] | [[Image:S14_M2D4_WF-Ylw.JPG | right |thumb|250px | W/F Gel, Yellow group]] |
Latest revision as of 07:27, 2 April 2014
DNA gels
T/R
W/F
Recall that lane 10 is singly digested (with EcoRV) DNA prepared by Su for a size comparison to the full-length plasmid versus plasmid with no insert.
Westerns
T/R
W/F
Hi folks -- I think these got flipped because they were sent from my phone. Please turn them in the usual orientation (and crop) for use in your reports.