BME494 Project Group9: Difference between revisions
Line 114: | Line 114: | ||
* '''Future Vision''': D-Vitameter | * '''Future Vision''': D-Vitameter | ||
Ideally this would be a device that would benefit from the proposed technology. This compact and portable device would allow patients to get a reading of their ability to produce Vitamin D by just using one drop of blood. The basic components of this small device would be an enclosed box with a small polycarbonate window. The device will allow to introduce a sample in a microscope slide and shine a UV light to detect presence of 25(OH). | Ideally this would be a device that would benefit from the proposed technology. This compact and portable device would allow patients to get a reading of their ability to produce Vitamin D by just using one drop of blood. The basic components of this small device would be an enclosed box with a small polycarbonate window. The device will allow to introduce a sample in a microscope slide and shine a UV light to detect presence of 25(OH). | ||
[[Image:Dvitameter.jpg|600px]] | [[Image:Dvitameter.jpg|600px]] | ||
Illustration of D-Vitameter. | Illustration of D-Vitameter. | ||
<br><br><br><br><br> | <br><br><br><br><br> |
Revision as of 23:45, 15 March 2012
Home People Course Projects Course Materials Syllabus Photos Wiki Editing Help
ABSTRACT
BACKGROUND
PROOF OF CONCEPT DESIGNIn the presence of 25(OH)D3 in blood, the co-transcription factor and promoter for the CYP27B1 are activated. When the promoter is activated, it expresses the enzyme that causes a chemical change to the active form of Vitamin D. This active form, 1-25-DiHydroxyVitamin D3, is created in the kidney. If the promoter is activated, then it will express the enzyme that chemically changes to the active form of vitamin D in the kidney.
The genes for the CYP27B1 co-transcription factors and promoter were provided via GenBank (http://www.ncbi.nlm.nih.gov/genbank/). Prior to primer creation, it was determined if there was the need for site directed mutagenesis. Fortunately, through the aid of Nebcutter, it was determined that the original gene set was ok to use as is. The forward primer: 5'-ctttctttcgcgagcacc-3' The reverse primer: 5'-tataaacgcagaaaggc-3' One pre-determined complication with this primer design is the lack for GC clamps. In testing, this could present future problems during transformation and amplification.
Assembly Scheme
Ampicillin and Kanamycin are utilized to help select for the appropriate plasmids durring testing.
Ideally this would be a device that would benefit from the proposed technology. This compact and portable device would allow patients to get a reading of their ability to produce Vitamin D by just using one drop of blood. The basic components of this small device would be an enclosed box with a small polycarbonate window. The device will allow to introduce a sample in a microscope slide and shine a UV light to detect presence of 25(OH). Illustration of D-Vitameter.
TESTING
Both samples will be run through a spectrophotometer to measure absorbency at wavelength peaks specific to GFP. A max peak of GFP absorbancy is expected around 395nm in addition to a minor absorbancy at 475nm.
When 25(OH)D3 is present, ideally there would be a 100% GFP concentration
HUMAN PRACTICES•The meter is practical because the blood sample is relatively small and can be extracted at any time. Normal tests for Vitamin D deficiency require laboratory procedures and time.
OUR TEAM
Senior Biomedical Engineering, SBHSE, I am interested in learning the general concepts of synthetic biology and its applications, I will be graduating this semester! http://openwetware.org/wiki/User:Carolina_Tostado
Senior in Biochemistry & Dance. I am excited to learn the role of biochemistry within the field of synthetic biology. I am also graduating this semester!
Junior in Biomedical Engineering, SBHSE.
|