Knight:Reconstituting primers: Difference between revisions
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===Long version=== | ===Long version=== | ||
<math>Y\ \mu L\ =\ \frac{1}{25\ \mu | <math>Y\ \mu L\ =\ \frac{1 L}{25\ \mu moles}*X\ nmoles\ primer\ =\ \frac{1*10^6\ \mu L}{25000\ nmoles}*X\ nmoles\ primer\ =\ 40\ *\ X\ nmoles\ primer</math> | ||
==Notes== | ==Notes== | ||
See [[Reconstituting primers]] for a lot of useful information including why you should use TE buffer rather than water for reconstituting primers. | See [[Reconstituting primers]] for a lot of useful information including why you should use TE buffer rather than water for reconstituting primers. | ||
[[Category:Protocol]] [[Category:DNA]] [[Category:In vitro]] | |||
Latest revision as of 06:12, 7 May 2010
Procedure
Invitrogen recommends the following reconstitution procedure -
- Centrifuge the tube for a few seconds to get all the DNA to the bottom of the tube.
- To make a 25 μM stock, add YμL of sterile H2O to X nmoles of dry primer stock. (See equations below).
- Allow to sit for 2 mins, then vortex for 15 secs.
Short version
[math]\displaystyle{ Y\ \mu L\ =\ 40\ *\ X\ nmoles\ primer }[/math]
The number of nmoles of material in the tube (X) should be listed on the pages accompanying your primer order.
Long version
[math]\displaystyle{ Y\ \mu L\ =\ \frac{1 L}{25\ \mu moles}*X\ nmoles\ primer\ =\ \frac{1*10^6\ \mu L}{25000\ nmoles}*X\ nmoles\ primer\ =\ 40\ *\ X\ nmoles\ primer }[/math]
Notes
See Reconstituting primers for a lot of useful information including why you should use TE buffer rather than water for reconstituting primers.
