User:Mary Mendoza/Notebook/CHEM 581: Experimental Chemistry I/2014/10/24: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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== | ==Column Chromatography for BPA Concentration== | ||
* | * The column used was a 30 cc plastic syringe. The first layer was filled with glass wool fiber and fine sand. The sand was ground by Dr. Hartings using mortar and pestle. | ||
* 9.9999 g of BDH Silica Gel 60 Angstroms High Purity 40-63 μm was mixed with approximately 30 mL of methanol. | |||
[[Image:IMG 3210.JPG|thumb|center|600x400px|BDH Silica Gel 60 Angstroms High Purity 40-63 μm]] | |||
* First, the column was washed with water. Afterwards, the silica-methanol mixture was slowly poured to leave the sand layer undisturbed. | |||
* After some of the solvent front, methanol, passed, the column was washed with water again. Air pressure was applied to compact the column. | |||
* Another water wash was performed. | |||
* Once the column was ready, 10 mL BPA sample was poured into the column; solvent of the BPA was drained through the column. | |||
* When most of the sample solvent has been drained, 10 mL of water was added into the column. | |||
* 2 fractions of the 10 mL water and 6, 5 mL fractions of the 30 mL methanol were collected. Each fraction was dotted onto a thin layer chromatography (TLC) paper. | |||
* After dotting the TLC paper, there were no observed changes. Hence, Dr. Hartings dissolved FeCl<sub>3</sub> in methanol. FeCl<sub>3</sub> is expected to form a blue compound once added to phenolic compounds. However, no change was observed even with the addition of FeCl<sub>3</sub>. | |||
* The experiment was terminated. | |||
* No change observed could have been that the 10 ppm BPA is too dilute of a concentration for the successful, efficacy of the TLC method. | |||
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Latest revision as of 00:28, 27 September 2017
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Column Chromatography for BPA Concentration
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