User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/02/26: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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===Hybridization of molecular beacon and complementary strand at varying concentrations=== | ===Hybridization of molecular beacon and complementary strand at varying concentrations=== | ||
====MB:CS (MB at 500pM)==== | ====MB:CS (MB at 500pM)==== | ||
(7.3x10^-6M)V=(3x10^-6)(10μL) | (7.3x10^-6M)V=(3x10^-6)(10μL)<br.> | ||
4.1μL MB in 5.9μL buffer | 4.1μL MB in 5.9μL buffer | ||
<br.> | <br.> | ||
(2.8x10^-5)V=(3x10^-6M)(10μL) | (2.8x10^-5)V=(3x10^-6M)(10μL)<br.> | ||
1.1μL CS in 8.9μL buffer | 1.1μL CS in 8.9μL buffer | ||
<br.> | <br.> | ||
(3x10^-6)V=(500x10^-9)(50μL) | (3x10^-6)V=(500x10^-9)(50μL)<br.> | ||
8.33μL in 41.67μL | 8.33μL in 41.67μL | ||
<br.> | <br.> | ||
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*50:1 (50μL MB:1μL CS) - 0.98μM MB:0.02μM CS | *50:1 (50μL MB:1μL CS) - 0.98μM MB:0.02μM CS | ||
<br.> | <br.> | ||
Make 1nm, 500pM, and 100pM samples for each ratio | =====Make 1nm, 500pM, and 100pM samples for each ratio===== | ||
1:1 | |||
*50nM: (500nM)V=(50nM)(50μL) | |||
5μL 500nM solution in 45μL buffer | |||
*1nM: (50nM)V=(1nM)(50μL) | |||
1μL 50nM solution in 49μL buffer | |||
*500pM (0.5nM): (1nM)V=(0.5nM)(50μL) | |||
25μL 1nM solution in 25μL buffer | |||
*100pM (0.1nM): (0.5nM)V=(0.1nM)(50μL) | |||
10μL 0.5nM solution in 40μL buffer | |||
1:5, 1:10, 5:1, 10:1, and 50:1 calculations done in the same way | |||
Latest revision as of 22:33, 26 September 2017
Project name | Main project page Previous entry Next entry |
February 26, 2013Hybridization of molecular beacon and complementary strand at varying concentrationsMB:CS (MB at 500pM)(7.3x10^-6M)V=(3x10^-6)(10μL)<br.> 4.1μL MB in 5.9μL buffer <br.> (2.8x10^-5)V=(3x10^-6M)(10μL)<br.> 1.1μL CS in 8.9μL buffer <br.> (3x10^-6)V=(500x10^-9)(50μL)<br.> 8.33μL in 41.67μL <br.>
<br.> MB:CS (CS at 500pM)(7.3x10^-6)V=(5x10^-6)(5μL) 3.42μL MB in 1.57μL buffer <br.> 0.36μL CS in 9.64μL buffer
<br.> Make 1nm, 500pM, and 100pM samples for each ratio1:1
5μL 500nM solution in 45μL buffer
1μL 50nM solution in 49μL buffer
25μL 1nM solution in 25μL buffer
10μL 0.5nM solution in 40μL buffer 1:5, 1:10, 5:1, 10:1, and 50:1 calculations done in the same way
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