User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/10/16: Difference between revisions
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.56mg= 5600 ng | .56mg= 5600 ng | ||
M<sub>1</sub>V<sub>1</sub>= M<sub>2</sub>V<sub>2</sub> | M<sub>1</sub>V<sub>1</sub>= M<sub>2</sub>V<sub>2</sub> | ||
5600 ng/μL x V<sub>1</sub>= 100ng x 1000μL | 5600 ng/μL x V<sub>1</sub>= 100ng x 1000μL | ||
*(The container containing the plasmid only had room for 1mL of water to be added- as a result, a dilution needed to be done in order to get the appropriate concentration. | *(The container containing the plasmid only had room for 1mL of water to be added- as a result, a dilution needed to be done in order to get the appropriate concentration. 1mL of water was added to dissolve the plasmid prior to the dilution | ||
V<sub>2</sub>= 17.857μL is the amount of plasmid solution pipetted out in order to get a concentration of 100ng/μL | V<sub>2</sub>= 17.857μL is the amount of plasmid solution pipetted out in order to get a concentration of 100ng/μL | ||
Revision as of 16:40, 21 November 2012
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PCR of ADA MutationsGoals
Preparation
Concentration of plasmid needed for experiment= 100ng/μL
.56mg= 5600 ng M1V1= M2V2 5600 ng/μL x V1= 100ng x 1000μL
V2= 17.857μL is the amount of plasmid solution pipetted out in order to get a concentration of 100ng/μL Procedure
Calculations
Total Volume in cuvet= 1500μL Conclusions
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