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  • We attempted to extract the red florescent biobrick dna containing plasmid by following the bio brick procedure.
  • The biobrick was not successfully isolated as no bacterial colonies grew.
  • We prepped our primers for PCR and ran a gradient of temperatures, 44,47.8,51.4,56.2,59.9,64 in the PCR machine to discover our ideal annealing temperature.
  • Primers were left to run the PCR cycle and then put in the -20 freezer.