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Human DNA extraction and Amplification

  • Today we used the Qiagen Kit to extract DNA from our cheek cells and hair follicles.
  • We then amplified a section of the DNA (the ALU repeat portion referred to in other journals) using a PCR protocol and materials taken from the kit.
  • In preparation for adding this DNA section to pBluescript we also digested a sample of that vector.
  • Next time we will verify the results of this lab using electro-phoresis.