840:153g:Projects/project27/2012/11/15

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Transformation-Take 2

  • The ligation products from 11/8 were used to transform competent E. coli cells. Two ligations were performed for both the pSB1K3 and J61002 vectors, with the insert being the J23100 promoter. Each vector was therefore transformed into two different volumes of competent cells, making four total transformations with additional positive and negative controls. The known vector pSB1A7, carrying ampicillin resistance, was used as the positive control. For a negative control, cells were transformed with water. After transformation, cells were plated on growth media containing appropriate antibiotics (ampicillin for J23100 and kanamycin for PSB1K3) and placed in the 37°C incubator.